UNC 0631

SKU: orb1703912

Description

UNC 0631 is a potent and selective small-molecule inhibitor of the histone methyltransferase G9a (EHMT2), with an IC50 of 4 nM. It is widely used in epigenetic research to probe the role of H3K9 methylation in gene silencing, cancer biology, and cellular differentiation in both in vitro and in vivo models.

Research Area

Epigenetics & Chromatin

Key Properties

• CAS Number: 1320288-19-4
• MW: 635.93
• Purity: 99.92%
• Formula: C₃₇H₆₁N₇O₂
• SMILES: COc1cc2c(NC3CCN(CC4CCCCC4)CC3)nc(nc2cc1OCCCN1CCCCC1)N1CCCN(CC1)C(C)C
• Target: Histone Methyltransferase
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (5.19 mM);Ethanol:5 mg/mL (7.86 mM);DMSO:49 mg/mL (77.05 mM)

Bioactivity

• Target IC50:
  G9a:4 nM
• In Vivo:
  In MCF7,22RV1 and IMR90 cells, UNC0631 significantly reduced H3K9me2 levels.
• Cell Research:
  MDA-MB-231, PC3, HCT116 cells are cultured in RPMI with 10% FBS, 22RV1 cells in alphaMEM and 10% FBS, MCF7 and IMR90 cells in DMEM with 10% FBS. Cells are treated with inhibitors for 48 h. The media is removed and replaced with DMEM 10% FBS without phenol red supplemented with 1 mg/mL of MTT and incubated for 1–2 h. Live cells reduce yellow MTT to purple formazan. The resulting formazanis solubilized in acidified isopropanol and 1% Triton. Formazan signal absorbance is measured at 570 nm and corrected for the 650 nm background. IC50s are calculated using GraphPad Prizm statistical package with sigmoidal variable slope dose response curve fit.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

chromatin, cell-penetration, inhibit, p53, KMT1D, Inhibitor, HistoneMethyltransferase, Histone Methyltransferase, EHMT1, G9a, H3K9me2, PKMT, UNC 0631, UNC0631, UNC-0631

Compound Identifiers

PubChem CID

53315868