UMI-77

SKU: orb1301132

Description

UMI-77

Research Area

Cell Biology

Key Properties

• CAS Number: 518303-20-3
• MW: 468.34
• Purity: 99.57%
• Formula: C₁₈H₁₄BrNO₅S₂
• SMILES: OC(=O)CSc1cc(NS(=O)(=O)c2ccc(Br)cc2)c2ccccc2c1O
• Target: Bcl-2 Family
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:8.6 mg/mL (18.36 mM);Ethanol:86 mg/mL (183.63 mM);DMSO:83.33 mg/mL (177.93 mM)

Bioactivity

• Target IC50:
  Bcl-xL:32.99 μM (Ki)|Bcl-W:8.19 μM (Ki)|Bfl-1:5.33 μM (Ki)|Bcl-2:23.83 μM (Ki)|MCL1:490 nM(Ki)
• In Vivo:
  METHODS: UMI-77 (60, 80 mg/kg, intravenous injection) was used to observe the growth of body weight tumors in the BxPC-3 xenograft model of SCID mice and perform Western blot and immunohistochemical analysis of tumor tissues. RESULTS Treatment with UMI-77 daily for 5 consecutive days per week for two weeks inhibited tumor growth by 65% ​​and 56% on day 19 and day 22, respectively; Western blot of tumor tissue lysates showed slightly increased levels of pro-apoptotic proteins Bax and Bak and significantly reduced survivin.
• In Vitro:
  METHODS: A panel of five PC cell lines (MiaPaCa-2, AsPC-1, Panc-1, BxPC-3, Capan-2) with different expression of anti-apoptotic and pro-apoptotic proteins were treated with UMI-77 (1, 10, 100 μM) and the cytotoxic effects were evaluated. RESULTS The IC50 values ​​of UMI-77 against BxPC-3, Panc-1, MiaPaCa-2, AsPC-1, and Capan-2 cell lines were (3.4, 4.4, 12.5, 16.1, 5.5 μM), respectively.
• Cell Research:
  Human pancreatic cancer cell lines AsPC-1, BxPC-3, and Capan-2 are cultured in RPMI-1640 medium, whereas Panc-1 and MiaPaCa are cultured in Dulbeccos' Modified Eagle's Medium (DMEM), all supplemented with 10% FBS. The cell growth inhibition after treatment with increasing concentrations of the compounds is determined by WST-8 assay.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, Mcl-1, Bcl-2 Family, UMI 77, UMI77, UMI-77

Compound Identifiers

PubChem CID

992586