ULK1 Antibody: Biotin

• Catalog Number: orb384045
• Category: Antibodies
• Description: Rabbit polyclonal antibody against ULK1 conjugated to Biotin.
• Target: ULK1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Peptide Affinity Purified
• Immunogen: Synthetic peptide from the mid-protein of Human ULK1 (aa. 567-577)
• UniProt ID: O75385
• MW: 100 kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Application notes: A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Unc-51 like kinase 1 (C. elegans) antibody, Autoph
• Note: For research use only
• Entrez: 8408
• NCBI: NP_003556.1

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK1 Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 112.6 kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa (15ug). Lane 3: Human 293Trap (15ug). Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.6 kDa.

Western blot analysis of Rat Brain cell lysates showing detection of ~112.6kDa ULK1 protein using Rabbit Anti-ULK1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 μg. Block: 2% GE Healthcare Blocker (RT, 60 minutes). Primary Antibody: Rabbit Anti-ULK1 Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1/2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~112.6kDa.