UHRF1 Antibody

• Catalog Number: orb1258198
• Category: Antibodies
• Description: UHRF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UHRF1 (NP_001041666.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 110kDa
• Target: UHRF1
• UniProt ID: Q96T88
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: UHRF1, ubiquitin-like, containing PHD and RING fin
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using UHRF1 antibody (orb1258198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human lung cancer using UHRF1 antibody (orb1258198) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using UHRF1 antibody (orb1258198) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using UHRF1 antibody (orb1258198) at dilution of 1:100 (40x lens).