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Catalog Number | orb1937526 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB31273 |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:500, IHC-P: 1:100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 61036 Da |
Target | This UGT2B15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human UGT2B15. |
UniProt ID | P54855 |
NCBI | NP_001067.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | UDP-glucuronosyltransferase 2B15, UDPGT 2B15, HLUG Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-UGT2B15 Antibody (Center) at 1:500 dilution + Human liver tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of UGT2B15 Antibody (Center) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
UGT2B15 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.