UCP2 Antibody

SKU: orb1246814

Description

UCP2 Antibody

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in Rat Adipose and 28-30kDa in Mouse Spleen lysates (calculated MW of 33.4kDa according to Mouse NP_035801.3 and 33.4kDa according to Rat NP_062227.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the Mitochondria of MCF7 cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DSVKQFYTKGSEH
• Target: UCP2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

UCP2, uncoupling protein 2 (mitochondrial, proton carrier), SLC25A8, UCPH , uncoupling protein 2, uncoupling protein-2

orb1246814 (1 ug/ml) staining of Rat Adipose (A) and Mouse Spleen (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246814 (3.75 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1246814 (3.75 ug/ml) staining of paraffin embedded Human Adipocytes. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining. This data is from a previous batch, not on sale.

orb1246814 (3.75 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.

orb1246814 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI.

Quick Database Links

Gene Symbol

UCP2

UniProt

P55851

RefSeq (Protein):NP_003346.2