UCK Antibody (C-term)

• Catalog Number: orb1928583
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB3876
• Tested applications: IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 22222 Da
• Target: This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-162 amino acids from the C-terminal region of human UCK.
• UniProt ID: P30085
• NCBI: NP_057392.1, NP_001129612.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: UMP-CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172},
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The anti-UCK Pab is used in Western blot to detect UCK in HepG2 cell lysate (Lane 1) and mouse cerebellum tissue lysate (Lane 2).

Anti-UCK Antibody (L148) at 1:1000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.