UCHL3 Antibody (C-term)

SKU: orb1931711

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse, Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD6309
• Immunogen: This UCHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-225 amino acids from the C-terminal region of human UCHL3. Antigen Region: 195-225 aa.
• Target: UCHL3
• Molecular Weight: 26183 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCH-L3, Ubiquitin thioesterase L3, UCHL3

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with UCHL3 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with UCHL3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of UCHL3 in HepG2 cell line lysates (35 ug/lane). UCHL3 (arrow) was detected using the purified Pab.

Western blot analysis of his tag antibody in UCHL3 gene transfected 293 lysates (35 ug/lane). His-tag UCHL3 protein (arrow) is detected.

Western blot analysis of lysates from 293, 293T, SW620, U-87 MG cell line (from left to right), using UCHL3 Antibody (C209). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Quick Database Links

Gene Symbol

UCHL3

UniProt

P15374

RefSeq (Protein):NP_001257881.1, NP_005993.1