UBP43/USP18 Antibody

• Catalog Number: orb1535445
• Category: Antibodies
• Description: UBP43/USP18 Antibody
• Target: UBP43 / USP18
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.93 mg/ml
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Purification: Affinity purified
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.
• Tested applications: ELISA, IHC-P, WB
• Dilution range: ELISA (1:10000 - 1:50000), IHC-P (5 µg/ml), WB (1:500 - 1:2000)
• Application notes: Further information: Immunohistochemistry: LS-B1182 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1182 was determined to be 5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: USP18, 43 kDa ISG15-specific protease, ISG43, HUBP
• Note: For research use only