Ubiquitin-protein ligase E3A Antibody / UBE3A

• Catalog Number: orb1151594
• Category: Antibodies
• Description: Mouse monoclonal antibody to Ubiquitin-protein ligase E3A
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-UBE3A-1A2
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant full-length human UBE3A protein was used as the immunogen for the Ubiquitin-protein ligase E3A antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Ubiquitin-protein ligase E3A antibody is available for research use only.
• UniProt ID: Q05086
• Storage: Aliquot the Ubiquitin-protein ligase E3A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Ubiquitin-protein ligase E3A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells using Ubiquitin-protein ligase E3A antibody (green, clone PCRP-UBE3A-1A2) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with Ubiquitin-protein ligase E3A antibody (blue, clone PCRP-UBE3A-1A2), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Ubiquitin-protein ligase E3A antibody (clone PCRP-UBE3A-1A2). These results demonstrate the foremost specificity of the PCRP-UBE3A-1A2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Ubiquitin-protein ligase E3A antibody (PCRP-UBE3A-1A2) as confirmation of integrity and purity.