Ubiquitin Antibody: HRP

• Catalog Number: orb699407
• Category: Antibodies
• Description: Mouse monoclonal antibody against Ubiquitin conjugated to HRP
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FK1
• Tested applications: ELISA, IHC, WB
• Reactivity: All
• Isotype: IgM
• Immunogen: Ubiquitin conjugated lysozyme
• Concentration: 1 mg/ml
• Dilution range: WB (1:10000), ICC/IF (1:100)
• Conjugation: HRP
• Target: Ubiquitin
• Entrez: 7314
• UniProt ID: P0CG47
• NCBI: BAC56955.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: Polyubiquitin B antibody, RPS27A antibody, UBA52 a
• Note: For research use only
• Application notes: A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.

Western Blot analysis of Poly-Ubiquitin showing detection of Ubiquitin protein using Mouse Anti-Ubiquitin Monoclonal Antibody, Clone FK1. Lane 1: MW ladder. Lane 2: Poly-Ubiquitin, K48-linked (2 ug). Lane 2: Poly-Ubiquitin, K63-linked (0.6 ug). Load: 5 uL. Block: 1% BSA in TBST. Primary Antibody: Mouse Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:2000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.