Ubiquitin Antibody: APC

• Catalog Number: orb151478
• Category: Antibodies
• Description: Rabbit polyclonal to Ubiquitin (APC). Ubiquitin is a small protein that occurs in all eukaryotic Ubiquitin is a small protein that occurs in all eukaryotic...
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC
• Reactivity: Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Yeast
• Immunogen: Native bovine Ubiquitin, conjugated to KLH
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:100), ICC/IF (1:100)
• Conjugation: APC
• MW: 10kDa
• Target: Ubiquitin
• Entrez: 281370
• UniProt ID: P0CG53
• NCBI: NP_776558.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Alternative names: Polyubiquitin B antibody, RPS27A antibody, UBA52 a
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-119 was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysate showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody at 1:1000.

Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.

Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.