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Item 1 of 5
UBE4B Antibody (N-term)
Catalog Number: orb1931729
| Catalog Number | orb1931729 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB4133 |
| Tested applications | IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 146185 Da |
| Target | This UBE4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-142 amino acids from the N-terminal region of human UBE4B. |
| UniProt ID | O95155 |
| NCBI | NP_001099032.1, NP_006039.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Ubiquitin conjugation factor E4 B, 632-, UBE4B (HG Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded human testis tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
The anti-UBE4B Pab is used in Western blot to detect UBE4B in mouse kidney tissue lysate.
All lanes: Anti-UBE4B Antibody (N-term) at 1:1000 dilution. Lane 1: Raji whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 146 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-UBE4B Antibody (N-term) at 1:1000 dilution. Lane 1: K562 whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 146kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
