UBE4A Antibody (Center)

SKU: orb1935588

Description

UBE4A Antibody (Center)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 122561 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

UBE4A Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35 ug/lane). This demonstrates the UBE4A antibody detected the UBE4A protein (arrow).

All lanes: Anti-UBE4A Antibody (Center) at 1:1000 dilution. Lane 1: Caco2 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 123 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

UBE4A Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of UBE4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

UniProt

Q14139