UBE3A (C) Antibody, Rabbit Polyclonal

SKU: orb131782

Description

Rabbit polyclonal to UBE3A. Ubiquitin-protein ligase E3A (UBE3A) is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. UBE3A catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, UBE3A may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo.

Images & Validation

• Tested Applications: WB
• Dilution range: WB:1:1000-1:3000
• Reactivity: Human
• Application Notes:
  WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; ; Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of human UBE3A.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: C-terminal region of human UBE3A
• Molecular Weight: 101
• Purity: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Disclaimer: For research use only

Alternative Names

anti Ubiquitin-protein ligase E3A antibody, anti E6AP ubiquitin-protein ligase antibody, anti Human papillomavirus E6-associated protein antibody, anti Oncogenic protein-associated protein E6-AP antibody, anti Renal carcinoma antigen NY-REN-54 antibody, anti E6AP antibody, anti EPVE6AP antibody, anti HPVE6A antibody

Quick Database Links

UniProt

Q05086