UBE2U Antibody

SKU: orb1255878

Description

UBE2U Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human UBE2U (NP_689702.1).
• Target: UBE2U
• Molecular Weight: Observed: 42kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ubiquitin-conjugating enzyme E2 U, E2 ubiquitin-conjugating enzyme U, testicular tissue protein Li 216, ubiquitin carrier protein U, ubiquitin conjugating enzyme E2 U (putative), ubiquitin conjugating enzyme E2U (putative), ubiquitin-protein ligase U

Western blot analysis of extracts of various cell lines, using UBE2U antibody (orb1255878) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human liver using UBE2U antibody (orb1255878) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using UBE2U antibody (orb1255878) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

UBE2U

UniProt

Q5VVX9