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Description
Images & Validation
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| Tested Applications | WB |
|---|---|
| Dilution Range | WB: 1:500, WB: 1:2000 |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This UBE2D2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 44-76 amino acids from the N-terminal region of human UBE2D2. Antigen Region: 44-76 aa. |
| Target | UBE2D2 |
| Molecular Weight | 16735 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Ubiquitin-conjugating enzyme E2 D2, Ubiquitin carrier protein D2, Ubiquitin-conjugating enzyme E2(17)KB 2, Ubiquitin-conjugating enzyme E2-17 kDa 2, Ubiquitin-protein ligase D2, p53-regulated ubiquitin-conjugating enzyme 1, UBE2D2, PUBC1, UBC4, UBC5B, UBCH4, UBCH5B
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Western blot analysis of lysates from Hela, U-87 MG, MCF-7, SW480 cell line and rat testis tissue (from left to right), using UBE2D2 Antibody (N-term). Diluted at 1:500 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.
All lanes: Anti-UBE2D2 Antibody (N-term) at 1:2000 dilution. Lane 1: F9 whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: SW480 whole cell lysate. Lane 4: 293 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
UBE2D2
UniProt
UniProt Details
− No UniProt data available
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UBE2D2 Antibody (N-term) (orb1788352)
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