You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1258371 |
---|---|
Category | Antibodies |
Description | UBE2D1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D1 (NP_003329.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 14kDa |
Target | UBE2D1 |
UniProt ID | P51668 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | UBE2D1, E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using UBE2D1 antibody (orb1258371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.
Immunohistochemistry of paraffin-embedded rat brain using UBE2D1 antibody (orb1258371) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using UBE2D1 antibody (orb1258371) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using UBE2D1 antibody (orb1258371) at dilution of 1:100 (40x lens).
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, IP, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
Filter by Rating