UBASH3A Antibody

SKU: orb1248862

Description

UBASH3A Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in Human Spleen lysates (calculated MW of 69.8kDa according to NP_001001895.1). Recommended concentration: 1-3µg/mL.Immunohistochemistry:In paraffin embedded Human Breast shows perinuclear staining in the epithelial cells of the lubules. Recommended concentration, 3-5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DSGIRISSVFASP
• Target: UBASH3A
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UBASH3A, ubiquitin associated and SH3 domain containing A, CLIP4, STS-2, TULA, OTTHUMP00000109368, T-cell ubiquitin ligand protein, cbl-interacting protein 4, suppressor of T-cell receptor signaling 2, ubiquitin-associated and SH3 domain-containing protein A

orb1248862 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248862 (3.8 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

UBASH3A

UniProt

P57075

RefSeq (Protein):NP_061834.1, NP_001001895.1