UBASH3A Antibody

• Catalog Number: orb1248862
• Category: Antibodies
• Description: UBASH3A Antibody
• Target: UBASH3A
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-DSGIRISSVFASP
• UniProt ID: P57075
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 70kDa band observed in Human Spleen lysates (calculated MW of 69.8kDa according to NP_001001895.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows perinuclear staining in the epithelial cells of the lubules. Recommended concentration, 3-5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: UBASH3A, ubiquitin associated and SH3 domain conta
• Note: For research use only
• NCBI: NP_061834.1, NP_001001895.1

orb1248862 (1 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248862 (3.8 ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.