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Tyrosinase Antibody / TYR
Catalog Number: orb1151865
| Catalog Number | orb1151865 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to Tyrosinase |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | TYR/3829 |
| Tested applications | FACS, IF, IHC-P |
| Reactivity | Human |
| Isotype | Mouse IgG1, kappa |
| Immunogen | A portion of amino acids 332-474 was used as the immunogen for the Tyrosinase antibody. |
| Antibody Type | Primary Antibody |
| Dilution range | Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
| Purity | Protein A/G affinity |
| Conjugation | Unconjugated |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Hazard Information | This Tyrosinase antibody is available for research use only. |
| UniProt ID | P14679 |
| Storage | Aliquot the Tyrosinase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
| Note | For research use only |
| Application notes | Optimal dilution of the Tyrosinase antibody should be determined by the researcher. |
| Expiration Date | 12 months from date of receipt. |
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Tyrosinase antibody (clone TYR/3829). These results demonstrate the foremost specificity of the TYR/3829 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC staining of FFPE human skin tissue with Tyrosinase antibody (clone TYR/3829). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Tyrosinase antibody (TYR/3829) as confirmation of integrity and purity.
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