Tyrosinase Antibody [OCA1/812]

• Catalog Number: orb1252455
• Category: Antibodies
• Description: Tyrosinase Antibody [OCA1/812]
• Target: TYR
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Purification: Protein G affinity chromatography
• Immunogen: Recombinant human protein was used as the immunogen for the Tyrosinase antibody.
• UniProt ID: P14679
• Tested applications: ELISA, FC, IF, IHC-P
• Application notes: ELISA (order Ab without BSA)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format: incubate for 30 min at RT (2)Optimal dilution of the Tyrosinase antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, or 10mM Citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: OCA1/812
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tyrosinase, LB24-AB, Monophenol monooxygenase, SK2
• Research Area: Cancer Biology, Metabolism Research, Signal Transd
• Note: For research use only

Formalin-fixed, paraffin-embedded melanoma stained with Tyrosinase antibody (OCA1/812).