Type IV Collagen Antibody

• Catalog Number: orb1251418
• Category: Antibodies
• Description: Type IV Collagen Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Canine, Equine, Gallus, Guinea pig, Human, Mammal, Mouse, Porcine, Primate, Rabbit, Rat, Rodent, Sheep
• Form/Appearance: Liquid
• Concentration: 0.4 mg/mL
• Buffer/Preservatives: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
• Purification: Purified by affinity chromatography on type IV collagen covalently linked to agarose; cross-adsorbed against Collagen types I, II, III, V, and VI for minimal reactivity
• Immunogen: Type IV collagen
• Tested applications: ELISA
• Application notes: Type IV Collagen Antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, flow cytometry, western blotting, and immunoprecipitation assays.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only

Human gastric cancer tissue was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by Swine Anti-Goat IgG (H + L), Human/Rat/Mouse SP ads-HRP.

Frozen sections of E12.5 eyes from Col4a1 + / + mice were stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by a secondary antibody and DAPI.

Itga3-/-/Gpr56-/- E11.5 mouse neocortex section was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418), ant-Tuj1, and anti-Nestin followed by secondary antibodies.

Frozen human nasal mucosa explant section 24 h post infection with HSV1 was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) and anti-HSV1 gD followed by a secondary reagents.

Frozen C57BL/6 mouse quadricep muscle section was stained with Goat Anti-Type IV Collagen-UNLB (Cat. No. orb1251418) followed by a secondary antibody.