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| Catalog Number | orb1251420 |
|---|---|
| Category | Antibodies |
| Description | Type III Collagen Antibody |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Bovine, Canine, Equine, Gallus, Guinea pig, Hamster, Human, Mammal, Mouse, Porcine, Primate, Rabbit, Rat, Rodent |
| Form/Appearance | Liquid |
| Concentration | 0.4 mg/mL |
| Buffer/Preservatives | Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added |
| Purification | Purified by affinity chromatography on type III collagen covalently linked to agarose; cross-adsorbed against Collagen types I, II, IV, V, and VI for minimal reactivity |
| Immunogen | Type III collagen |
| Tested applications | ELISA |
| Application notes | Type III Collagen Antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, flow cytometry, and western blotting assays. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Research Area | Signal Transduction |
| Note | For research use only |

Human gastric cancer tissue was stained with Goat Anti-Type III Collagen-UNLB (Cat. No. orb1251420) followed by Swine Anti-Goat IgG (H + L), Human/Rat/Mouse SP ads-HRP.

Frozen osteopontin-null mouse muscle section was stained with Goat Anti-Type III Collagen-UNLB (Cat. No. orb1251420) followed by a secondary antibody.

Rat kidney section was stained with Goat Anti-Type III Collagen-UNLB (Cat. No. orb1251420) followed by a secondary antibody and DAB and hematoxylin.

Frozen kidney section following unilateral ureteral obstruction was stained with Goat Anti-Type III Collagen-UNLB (Cat. No. orb1251420) followed by a secondary antibody.

Paraffin embedded doxorubicin treated rat kidney section was stained with Goat Anti-Type III Collagen-UNLB (Cat. No. orb1251420) followed by a secondary antibody and DAB.
DOT, ELISA, IHC, IP, WB | |
Bovine, Human, Porcine | |
Rabbit | |
Polyclonal | |
Unconjugated |
DOT, ELISA, IHC, IP, WB | |
Bovine, Human, Porcine | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P | |
Bovine, Canine, Gallus, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Gallus, Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, WB | |
Bovine, Canine, Gallus | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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