Type II Collagen Antibody

SKU: orb1251422

Description

Research Area

Signal Transduction

Images & Validation

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Item 1 of 2

Tested Applications	WB
Reactivity	Bovine, Gallus, Human, Mouse, Porcine, Rabbit, Rat, Sheep
Application Notes	Type II Collagen Antibody for use in ELISA, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, and immunoprecipitation assays.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Type II collagen
Purification	Purified by affinity chromatography on type II collagen covalently linked to agarose; cross-adsorbed against Collagen types I, III, IV, V, and VI for minimal reactivity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration	0.4 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Mouse chondrocytes were stained with Goat Anti-Type II Collagen-UNLB (Cat. No. orb1251422) followed by Rabbit Anti-Goat IgG (H + L), Human SP ads-FITC.

Paraffin embedded Col2a1-Cre;Nppcflox/flox mouse tibial growth plate section was stained with Goat Anti-Type II Collagen-UNLB (Cat. No. orb1251422) followed by an HRP secondary antibody and DAB.

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Protocol Information

Western Blot (IB, immunoblot)

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