Tubercidin

SKU: orb1300150

Description

Tubercidin (Sparsomycin A) is a nucleoside antibiotic analog of adenosine that incorporates into nucleic acids to inhibit DNA and RNA polymerases, blocking replication and transcription. It is utilized in biochemical research for its antiviral and antifungal properties in both in vitro and cell-based studies.

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology

Key Properties

• CAS Number: 69-33-0
• MW: 266.25
• Purity: 98.66% (May vary between batches)
• Formula: C₁₁H₁₄N₄O₄
• SMILES: Nc1ncnc2n(ccc12)[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O
• Target: Influenza Virus,Antibacterial,Antibiotic,DNA/RNA Synthesis
• Solubility: H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:55 mg/mL (206.57 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.51 mM)

Bioactivity

• In Vivo:
  Tubercidin at the dose regimen (5 mg/kg everyday for 4 days) is lethal when used alone. Some of the studies show that the mortality from tubercidin is primarily due to hepatic and renal injuries and, to a lesser extent, damage to the pancreas. Coadministration of NBMPR-P at 25 mg/kg per day completely protects the mice (100% survival) from this lethal dose regimen of tubercidin.
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• In Vitro:
  Continuous exposure for 14 days to tubercidin alone is highly toxic to both human CFU-GM and BFU-E. The IC50s of tubercidin are 3.4±1.7 and 3.7±0.2 nM for CFU-GM and BFU-E, respectively. Tubercidin also has a direct dose-dependent inhibitory effect on myeloid and erythroid human bone marrow progenitor cells in vitro.
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• Cell Research:
  Human bone marrow cells were obtained from healthy volunteers. In the protection studies, cells are exposed to 10 nM of tubercidin in the presence or absence of various doses of NBMPR-P. Cells are continuously exposed to drugs for 14 days in a humidified atmosphere of 5% C02-95% air at 37°C. Colonies of CFU-GM (250 cells) and BFU-E (>30 hemoglobinized cells) are counted with an inverted microscope. (Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sparsomycin A, Tubercidin, RNASynthesis, RNA Synthesis, DNASynthesis, DNA/RNA Synthesis, DNA Synthesis, Bacterial, Antibiotic, 7-Deazaadenosine, inhibit, Influenza Virus, InfluenzaVirus, Inhibitor

Compound Identifiers

PubChem CID

6245