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Item 1 of 3
TUBB2B Antibody (N-term)
Catalog Number: orb1937852
| Catalog Number | orb1937852 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB31679 |
| Tested applications | IF, IHC-P, WB |
| Predicted Reactivity | Other, Porcine |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:4000, IHC-P-Leica: 1:2000, IF: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 49953 Da |
| Target | This TUBB2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human TUBB2B. |
| UniProt ID | Q9BVA1 |
| NCBI | NP_821080.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Tubulin beta-2B chain, TUBB2B Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-TUBB2B Antibody (N-term) at 1:4000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: MDA-MB-231 whole cell lysate. Lane 4: K562 whole cell lysate. Lane 5: Mouse brain tissue lysate. Lane 6: Mouse cerebellum tissue lysate. Lane 7: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of TUBB2B Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of paraffin-embedded Human brain tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.
