TSQ

SKU: orb1944591

Description

TSQ is a zinc-specific fluorescent probe (λmax ~470 nm) used to image and chelate zinc in biological systems. Its fluorescence is quenched by the chelator TPEN. This tool is widely applied in both in vitro and in vivo research to study zinc homeostasis, apoptosis, and zinc-protein interactions.

Key Properties

• CAS Number: 109628-27-5
• MW: 328.39
• Purity: 99.69% (May vary between batches)
• Formula: C₁₇H₁₆N₂O₃S
• SMILES: COc1cc(NS(=O)(=O)c2ccc(C)cc2)c2ncccc2c1
• Target: Others
• Solubility: DMSO:80 mg/mL (243.61 mM)

Bioactivity

• Cell Research:
  Instructions: a. Reagent preparation: Prepare TSQ fluorescent probe solution according to the experimental purpose. b. Operation steps: 1. Culture the cells to be tested to an appropriate density and wash the cells with PBS buffer. 2. Add an appropriate amount of TSQ fluorescent probe solution to a final concentration that depends on the cell type and experimental requirements, generally 1-10 μM. 3. Incubate in a 37°C, 5% CO₂ incubator for 15-60 minutes. 4. After incubation, wash the cells with PBS buffer to remove unbound TSQ probes. 5. Observe the cells under a fluorescence microscope, set the excitation wavelength near the maximum excitation wavelength of TSQ, such as around 470 nm, and detect the fluorescence signal of zinc ions in the cells.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

130606