TSPYL6 Antibody

• Catalog Number: orb1270250
• Category: Antibodies
• Description: TSPYL6 Antibody
• Target: TSPYL6
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This TSPYL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-235 amino acids from the Central region of human TSPYL6.
• UniProt ID: Q8N831
• MW: 46 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Testis-specific Y-encoded-like protein 6, TSPY-lik
• Note: For research use only
• NCBI: Q8N831

TSPYL6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of TSPYL6 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis in NCI-H460, Hela cell line lysates (35 ug/lane).