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TSPAN2 Antibody (Center)
Description
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution range | WB - 1:1000, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 24148 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |

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TSPAN2 Antibody (Center) western blot analysis in CEM, K562, HL-60 cell line lysates (35 ug/lane).This demonstrates the TSPAN2 antibody detected the TSPAN2 protein (arrow).

TSPAN2 Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-TSPAN2 Antibody (Center) at 1:1000 dilution + K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 24 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
TSPAN2 Antibody (Center) (orb1927982)
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