TSN Antibody (Center)

SKU: orb1937525

Description

TSN Antibody (Center)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB - 1:1000, IF - 1:10-50, IHC-P - 1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Hamster

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 26183 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Fluorescent confocal image of Hela cell stained with TSN Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.

TSN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

TSN Antibody (Center) western blot analysis in HL-60, Jurkat, K562 cell line mouse spleen tissue lysates (35 ug/lane). This demonstrates the TSN antibody detected the TSN protein (arrow).

Quick Database Links

UniProt

Q15631