TSN Antibody (Center)

SKU: orb1937525

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

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Item 1 of 3

Tested Applications	IF, IHC-P, WB
Dilution Range	IF - 1:10-50, WB - 1:1000, IHC-P - 1:10-50
Reactivity	Human, Mouse
Predicted Reactivity	Hamster

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD53406
Immunogen	This TSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human TSN. Antigen Region: 109-138 aa.
Target	TSN (HGNC:12379)
Molecular Weight	26183 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Translin, 31--, Component 3 of promoter of RISC, C3PO, TSN

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Fluorescent confocal image of Hela cell stained with TSN Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.

TSN Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

TSN Antibody (Center) western blot analysis in HL-60, Jurkat, K562 cell line mouse spleen tissue lysates (35 ug/lane). This demonstrates the TSN antibody detected the TSN protein (arrow).