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Catalog Number | orb1256779 |
---|---|
Category | Antibodies |
Description | TSHR Antibody |
Target | TSHR |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 21-253 of human TSHR (NP_001018046.1). |
UniProt ID | P16473 |
MW | Observed: 110kDa |
Tested applications | IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | TSHR, LGR3, MGC75129, hTSHR-I, CHNG1 |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using TSHR antibody (orb1256779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (orb1256779) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using TSHR Antibody (orb1256779) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TSHR Antibody (orb1256779) at dilution of 1:100 (40x lens).
ELISA, WB | |
Bovine, Human | |
Goat | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |