TSC22D3 Antibody

• Catalog Number: orb1256780
• Category: Antibodies
• Description: TSC22D3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human TSC22D3 (NP_001015881.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 15kDa
• Target: TSC22D3
• UniProt ID: Q99576
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: TSC22D3 Antibody: DIP, GILZ, hDIP, DSIPI, TSC-22R,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TSC22D3 antibody (orb1256780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse lung using TSC22D3 Antibody (orb1256780) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using TSC22D3 antibody (orb1256780). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug TSC22D3 antibody (orb1256780). Western blot was performed from the immunoprecipitate using TSC22D3 antibody (orb1256780) at a dilution of 1:1000.