TSC22D3 Antibody

SKU: orb1256780

Description

TSC22D3 Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-79 of human TSC22D3 (NP_001015881.1).
• Target: TSC22D3
• Molecular Weight: Observed: 15kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

TSC22D3 Antibody: DIP, GILZ, hDIP, DSIPI, TSC-22R, TSC22 domain family protein 3, DSIP-immunoreactive peptide, Protein DIP

Western blot analysis of extracts of various cell lines, using TSC22D3 antibody (orb1256780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse lung using TSC22D3 Antibody (orb1256780) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using TSC22D3 antibody (orb1256780). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug TSC22D3 antibody (orb1256780). Western blot was performed from the immunoprecipitate using TSC22D3 antibody (orb1256780) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

TSC22D3

UniProt

Q99576