TSC1 Rabbit pAb Discontinued

SKU: orb136450

Description

Rabbit polyclonal to Hamartin

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution Range: WB: 1:500-1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human TSC1 (NP_000359.1).
• Molecular Weight: 150kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TSC1 antibody

Immunofluorescence analysis of NIH-3T3 cells using TSC1 Rabbit pAb (orb136450) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using TSC1 Rabbit pAb (orb136450) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse pancreatic islet using TSC1 Rabbit pAb (orb136450) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using TSC1 Rabbit pAb (orb136450) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Western blot analysis of lysates from Rat spleen, using TSC1 Rabbit pAb (orb136450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Quick Database Links

UniProt

Q92574

Zheng, Zu-Guo et al. Anhydroicaritin improves diet-induced obesity and hyperlipidemia and alleviates insulin resistance by suppressing SREBPs activation Biochem Pharmacol, 122, 42-61 (2016)

PubMed: 27816546