Trypsin Antibody Peroxidase Conjugated

SKU: orb344738

Description

Trypsin antibody (Peroxidase)

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, WB: 1:100 - 1:500
• Reactivity: Bovine
• Application Notes:
  Anti-Trypsin Peroxidase Antibody has been tested by ELISA and dot blot and is suitable for western blotting. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
• Purity: Trypsin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other sources is unknown.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-trypsin antibody HRP conjugation, peroxidase conjugated rabbit anti-trypsin antibody, Anionic trypsinogen antibody, Beta trypsin antibody, Brain trypsinogen antibody, Cationic Trypsinogen antibody, Digestive Zymogen antibody, Mesotrypsin antibody, Mesotrypsinogen antibody

Dot Blot results of Rabbit Anti-Trypsin Peroxidase Conjugated. Antigen: Trypsin. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Trypsin HRP 10 µg/ml for 1hr at RT. Secondary Antibody: none.

Quick Database Links

UniProt

P00760

RefSeq:CAA38513.1