Trypsin Antibody Biotin Conjugated

• Catalog Number: orb344766
• Category: Antibodies
• Description: Trypsin antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Anti-Trypsin Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified.
• Antibody Type: Primary Antibody
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: Trypsin Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin [Bovine Pancreas]. Cross reactivity against Trypsin from other sources may occur but has not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P00760
• NCBI: CAA38513.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-trypsin antibody biotin conjugation, b
• Note: For research use only
• Application notes: Anti-Trypsin Biotin Antibody has been tested by ELISA and western blot. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Trypsin Antibody Biotin Conjugated. Lane 1: Trypsin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Trypsin Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 minutes at RT. Predicted/Observed size: 26 kDa, 26 kDa for Trypsin. Other band(s): autocatalytic fragment of Trypsin, ~10 kDa.