TRPM8 Antibody (C-term C940)

SKU: orb1928520

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD36642
• Immunogen: This TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 926-956 amino acids from the C-terminal region of human TRPM8. Antigen Region: 926-956 aa.
• Target: TRPM8
• Molecular Weight: 127685 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, LTrpC-6, LTrpC6, Transient receptor potential p8, Trp-p8, TRPM8, LTRPC6, TRPP8

Western blot analysis of TRPM8 (arrow) using rabbit polyclonal TRPM8 Antibody (C-term C940).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2).

All lanes: Anti-TRPM8 Antibody (C-term C940) at 1:500 dilution. Lane 1: LNCap whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 125kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-TRPM8 Antibody (C-term C940) at 1:1000 dilution. Lane 1: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 128kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-TRPM8 Antibody (C-term C940) at 1:1000 dilution. Lane 1: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M (H/L), Peroxidase conjugated at 1/2000 dilution.Observed band size: 125kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody (C-term C940), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

TRPM8

UniProt

Q7Z2W7

RefSeq (Protein):NP_076985.4