TRMT2A Antibody

• Catalog Number: orb1257232
• Category: Antibodies
• Description: TRMT2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human TRMT2A (NP_073564.3).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: TRMT2A
• UniProt ID: Q8IZ69
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: HTF9C, tRNA (uracil-5-)-methyltransferase homolog
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using TRMT2A antibody (orb1257232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using TRMT2A antibody (orb1257232) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophagus using TRMT2A antibody (orb1257232) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse stomach using TRMT2A antibody (orb1257232) at dilution of 1:100 (40x lens).