TrkB phospho Tyr816/Y817 Antibody

SKU: orb3167682

Description

Our Anti-TrkB, phospho Tyr816/Y817 (TrkB, pY816/817) rabbit polyclonal primary antibody detects chicken (predicted), human, mouse, and rat (predicted) TrkB, phospho Tyr816/Y817 (TrkB, pY816/817), and is affinity-purified. It is validated for use in WB.

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 0.5-2 µg/mL
• Reactivity: Gallus, Human, Mouse, Rat
• Predicted Reactivity: Gallus, Rat
• Application Notes:
  High skim milk concentration (5%) causes suppression of pY817 signal, suggesting that 5% skim milk should be avoided as blocking buffer and antibody diluent. Strongest signal is obtained in 5% BSA blocking buffer, however, many non-specific bands are present. An equal mixture of skim milk and BSA (2.5% each) appears to provide the best compromise between signal and noise. However, optimization of blocking condition is recommended for each particular sample, with excess of BSA over skim milk likely to be beneficial for best results.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide immunogen, AKASPV[pY]LDILG
• Target: TrkB, phospho Tyr816/Y817 (TrkB, pY816/817)
• Molecular Weight: 120 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: PBS, pH 7.2-7.6, 0.1% trehalose, without preservatives.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; pTrkB; phosphoTrkB; phospho-TrkB

Quick Database Links

Gene Symbol

TrkB, phospho Tyr816/Y817 (TrkB, pY816/817)

UniProt

Q16620