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Catalog Number | orb1256643 |
---|---|
Category | Antibodies |
Description | TRIP10 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 246-545 of human TRIP10 (NP_004231.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 90kDa |
Target | TRIP10 |
UniProt ID | Q15642 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | TRIP10, STP, CIP4, HSTP, STOT, TRIP-10 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using TRIP10 antibody (orb1256643) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using TRIP10 antibody (orb1256643). Blue: DAPI for nuclear staining.
ELISA, ICC, IF, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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