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Item 1 of 6
Item 1 of 6
TriMethyl-Histone H3-K4 pAb
Catalog Number: orb1258191
| Catalog Number | orb1258191 |
|---|---|
| Category | Antibodies |
| Description | TriMethyl-Histone H3-K4 pAb |
| Target | H3K4me3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Other, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3 |
| UniProt ID | Q16695 |
| MW | Observed: 18kDa |
| Tested applications | ChIP, IF, IHC, IP, WB |
| Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:20 - 1:100CHIP seq: 1:20 - 1:100 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | H3F3A, H3t, H3.4, H3/g, H3FT |
| Note | For research use only |
Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K4 antibody (orb1258191). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (orb1258191) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K4 antibody (orb1258191) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using TriMethyl-Histone H3-K4 antibody (orb1258191) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K4 antibody (orb1258191). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody (orb1258191) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
