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H3K4me3 Antibody
SKU: orb1258191
Featured
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 6
| Tested Applications | ChIP, IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Other, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3 |
| Target | H3K4me3 |
| Molecular Weight | Observed: 18kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−H3F3A, H3t, H3.4, H3/g, H3FT
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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K4 antibody (orb1258191). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K4 antibody (orb1258191) at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using TriMethyl-Histone H3-K4 antibody (orb1258191) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human rectal cancer using TriMethyl-Histone H3-K4 antibody (orb1258191) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using TriMethyl-Histone H3-K4 antibody (orb1258191). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of 293 cell line, using TriMethyl-Histone H3-K4 antibody (orb1258191) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Quick Database Links
Gene Symbol
H3K4me3
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
ChIP
Chromatin Immunoprecipitation
H3K4me3 Antibody (orb1258191)
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