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Catalog Number | orb1927363 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB43552 |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | WB: 1:1000, WB: 1:2000, WB: 1:2000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 56400 Da |
Target | This TRIM6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human TRIM6. |
UniProt ID | Q9C030 |
NCBI | NP_477514.1, NP_001003818.1, NP_001185573.1, NP_001185574.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Tripartite motif-containing protein 6, RING finger Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
TRIM6 Antibody (N-term) western blot analysis in 293, A549, Ramos cell line lysates (35 ug/lane).This demonstrates the TRIM6 antibody detected the TRIM6 protein (arrow).
All lanes: Anti-TRIM6 Antibody (N-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-TRIM6 Antibody (N-term) at 1:2000 dilution. Lane 1: Ramos whole cell lysate. Lane 2: U-2OS whole cell lysate. Lane 3: PC-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.