TRIM50 Antibody (C-term)

• Catalog Number: orb1930421
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB27230
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 54728 Da
• Target: This TRIM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-383 amino acids from the C-terminal region of human TRIM50.
• UniProt ID: Q86XT4
• NCBI: NP_835226.2, NP_001268379.1, NP_001268380.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: E3 ubiquitin-protein ligase TRIM50, 632-, Triparti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

TRIM50 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

TRIM50 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TRIM50 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

TRIM50 Antibody (C-term) western blot analysis in Jurkat cell line lysates (15 ug/lane). This demonstrates the TRIM50 antibody detected the TRIM50 protein (arrow).