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Catalog Number | orb1248523 |
---|---|
Category | Antibodies |
Description | TRAP2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, WB |
Predicted Reactivity | Bovine, Canine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-VILRKNPNYDL |
Antibody Type | Primary Antibody |
Concentration | 500 ug/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | PSMD2 |
UniProt ID | Q13200 |
NCBI | NP_002799.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | PSMD2, TRAP2, MGC14274, S2, P97, proteasome (proso Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in lysates of cell lines HeLa, U251, NIH3T3 and KNRK (calculated MW of 100kDa according to Human NP_002799.3, Mouse NP_598862.1 and Rat NP_001026809.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1248523 (1 μg/ml) staining of HeLa (A), U251 (B), KNRK (C) and (0.3 ug/ml) NIH3T3 (D) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.
ELISA, FC, IF, WB | |
Bovine, Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |