PSMD2 Antibody

SKU: orb1248523

Description

PSMD2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, ICC, WB
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in lysates of cell lines HeLa, U251, NIH3T3 and KNRK (calculated MW of 100kDa according to Human NP_002799.3, Mouse NP_598862.1 and Rat NP_001026809.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-VILRKNPNYDL
• Target: PSMD2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PSMD2, TRAP2, MGC14274, S2, P97, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, 55.11 protein, TNFR-associated protein 2, 26S proteasome subunit p97, 26S proteasome regulatory subunit S2, 26S proteasome non-ATPase regulatory subunit 2, tumor necrosis factor receptor-associated protein 2, Rpn1, proteasome 26S non-ATPase subunit 2

orb1248523 (1 μg/ml) staining of HeLa (A), U251 (B), KNRK (C) and (0.3 ug/ml) NIH3T3 (D) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PSMD2

UniProt

Q13200

RefSeq (Protein):NP_002799.3