TRAP100 rabbit pAb Antibody

SKU: orb766514

Description

Rabbit polyclonal antibody to TRAP100

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MED24. AA range:801-850
• Molecular Weight: 110
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-MED24 antibody, anti-ARC100 antibody, anti-CRSP4 antibody, anti-DRIP100 antibody, anti-KIAA0130 antibody, anti-THRAP4 antibody, anti-TRAP100 antibody, anti-Mediator of RNA polymerase II transcription subunit 24 antibody, anti-Activator-recruited cofactor 100 kDa component antibody, anti-ARC100 antibody, anti-Cofactor required for Sp1 tran antibody

Western Blot analysis of various cells using TRAP100 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunofluorescence analysis of HUVEC cells, using MED24 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, using MED24 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).