Trap1 Antibody

• Catalog Number: orb181854
• Category: Antibodies
• Description: Mouse Anti-Human Trap1 Monoclonal IgG1 Kappa
• Target: TRAP1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1 Kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: Purified recombinant TRAP1
• UniProt ID: Q12931
• MW: 75kDa
• Tested applications: ICC, IF, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Application notes: 1 µg/ml of SMC-207 was sufficient for detection of Trap-1/HSP75 in 20 µg of Human A431 lysate by ECL immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Clone Number: 3H4-2H6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Heat shock protein 75 Antibody, Heat shock protein
• Research Area: Cancer Biology, Cell Biology, Protein Biochemistry
• Note: For research use only
• Entrez: 10131
• NCBI: NP_057376.2

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6. Tissue: Myoblast cell line C2C12. Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human, Rat Human A431 and Rat Brain Membrane cell lysates showing detection of ~75 kDa Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6. Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Rat lysate, Rat Brain Membrane (RBM). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Color Development: TMB solution for 15 min at RT. Predicted/Observed Size: ~75 kDa.