Transferrin Antibody

SKU: orb1925719

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research, Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:25, WB - 1:2000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This Transferrin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-135 amino acids from human Transferrin.
• Target: TF (HGNC:11740)
• Molecular Weight: 77064 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin, TF

All lanes: Anti-Transferrin Antibody at 1:2000 dilution. Lane 1: human plasma lysate. Lane 2: human fetal liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 77kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Transferrin Antibody at 1:2000 dilution. Lane 1: human plasma lysate. Lane 2: human liver lysate. Lane 3: human fetal liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 77 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Transferrin at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

TF (HGNC:11740)

UniProt

P02787