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Catalog Number | orb1257956 |
---|---|
Category | Antibodies |
Description | TRAF3 Antibody |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human TRAF3 |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 70kDa |
Target | TRAF3 |
UniProt ID | Q13114 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | TRAF3 Antibody: CAP1, LAP1, CAP-1, CRAF1, IIAE5, C Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using TRAF3 antibody (orb1257956) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using TRAF3 antibody (orb1257956). Blue: DAPI for nuclear staining.
IF, IH, IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |