TRAF3 Antibody

• Catalog Number: orb1257956
• Category: Antibodies
• Description: TRAF3 Antibody
• Target: TRAF3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic peptide of human TRAF3
• UniProt ID: Q13114
• MW: Observed: 70kDa
• Tested applications: IF, IHC, IP, WB
• Application notes: WB: 1:200 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TRAF3 Antibody: CAP1, LAP1, CAP-1, CRAF1, IIAE5, C
• Research Area: Cancer Biology, Cell Biology, Immunology & Inflamm
• Note: For research use only

Western blot analysis of extracts of various cell lines, using TRAF3 antibody (orb1257956) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using TRAF3 antibody (orb1257956). Blue: DAPI for nuclear staining.