TRADD Antibody

• Catalog Number: orb1269875
• Category: Antibodies
• Description: TRADD Antibody
• Target: TRADD
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This TRADD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-151 amino acids from the Central region of human TRADD.
• UniProt ID: Q15628
• MW: 34 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Tumor necrosis factor receptor type 1-associated D
• Research Area: Cancer Biology, Cell Biology, Immunology & Inflamm
• Note: For research use only
• NCBI: Q15628
• Expiration Date: 12 months from date of receipt.

Western blot analysis in Hela cell line lysates (35 ug/lane).

TRADD Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of TRADD Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).