TPSAB1 Antibody / Mast Cell Tryptase

• Catalog Number: orb1825298
• Category: Antibodies
• Description: Tryptases comprise a family of trypsin-like serine proteases (peptidase family S1). Tryptases are stored in mast cell secretory granules and basophils. Mast cells are connective tissue cells derived from blood-forming tissues that line arterial walls and secrete substances, which mediate inflammatory and immune responses. Tryptases are released into the extracellular environment and are resistant to all known endogenous proteinase inhibitors. This antibody reacts with mast cells distributed in skin, synovium, lung, and heart. This antibody does not bind with any other cell type. Human mast cell tryptase is considered to be an important marker of mast cell activation and is an important mediator of inflammation. Mastocytosis is a term collectively used for a group of disorders in which there is abnormal accumulation of mast cells in one or multiple organs. Anti-tryptase, combined with anti-CD2, anti-CD25, and anti-CD117, can be useful in identifying reactive mast cell hyperplasia, myelogenous neoplasms, mast cell leukemia, and mastocytosis.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human Tryptase 1 protein fragment (within amino acids 100-250) was used as the immunogen for the recombinant TPSAB1 antibody.
• UniProt ID: Q15661
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Antibody Type: Primary Antibody
• Clone Number: rTPSAB1/1963
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Storage: Aliquot the recombinant TPSAB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This recombinant TPSAB1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human tonsil tissue with recombinant TPSAB1 antibody (clone rTPSAB1/1963). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant TPSAB1 antibody (clone rTPSAB1/1963) as confirmation of integrity and purity.