TPD52 Antibody

• Catalog Number: orb1246845
• Category: Antibodies
• Description: TPD52 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-TEPLPEKTQES
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26-28kDa band observed in Human Lymph Node and Human Kidney lysates (calculated MW of 24.3kDa according to NP_001020423.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tiacci et al , Blood. 2005 Apr 1;105(7):2812-20.; PMID: 15576473). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: TPD52
• UniProt ID: P55327
• NCBI: NP_005070.1, NP_001020423.1, NP_001020424.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: TPD52, tumor protein D52, D52, N8L, PC-1, PrLZ, hD
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26-28kDa band observed in Human Lymph Node and Human Kidney lysates (calculated MW of 24.3kDa according to NP_001020423.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tiacci et al , Blood. 2005 Apr 1;105(7):2812-20.; PMID: 15576473). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246845 (1 ug/ml) staining of Human Lymph Node lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246845 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.